The usefulness of the reticulin stain in the differential diagnosis of liver nodules on fineneedle aspiration biopsy cell block preparations. Gordon and sweets technique demonstrates reticulin fibres using an argyrophiltype reaction involving silver solutions. Automated grading of reticulin stain of bone marrow. Problems and pitfalls in grading of bone marrow fibrosis, collagen deposition and osteosclerosis a consensus. Reticulin stain kit is a metal impregnation technique where. The value of this stain in detecting a hitherto unsuspected fungal infection is highlighted in the case described. Haematopoietic abnormalities associated with tacrolimus are relatively rare with reversible pure red cell aplasia being the most common. The trabecular plates are thin, and less than two cell in thickness. Demonstrates the reticular fibers in cirrhosis the fibers are disrupted. Gomori reticular fiber stain research 042016 powerpoint 1. An ammoniacal silver nitrate solution is applied to stain the reticulin fibers in tissue.
This is a helpful stain to use when the diagnosis of hepatocellular carcinoma is being entertained. Reticulin fibers are agyrophilic, meaning that these tissue elements will stain black with a silver solution using the aid of a chemical reducer, which brings the silver into a visible form. While it is not clear in rodent toxicity or preclinical studies if there is a need to distinguish among fibrosis types, much has been recently learned in humans regarding this topic. Dr lal pathlabs offers test service for histopathology special stain reticulin test for checking surgical pathology. This 10 component comprehensive reticulin stain kit is excellent stain for the identification of reticulin fiber in tissue sections. The reticulin stain demonstrates zellballen phenomenon in the. Determining the grade of reticulin fibrosis in the bone marrow is currently performed subjectively using manual and visual grade assignment by hematopathologists with unavoidable intra and interobserver bias. After a few days the solution begins to take on a pale yellowbrown color which may or may not become. In pathology, the reticulin stain, is a popular staining method in histology. Reticulum stain kit connective tissue stain ab150684. Reticular fibers characteristics can also aid in the diagnosis of certain tumors. Most studies have shown that absent or decreased reticulin stain or an abnormal reticulin pattern with widened trabeculae is reliable for the diagnosis of welldifferentiated hcc. Reticulinnuclear fast stain kit is used to identify a primitive form of connective tissue, called reticulin, in tissue sections on the artisan link and artisan link pro staining systems. Ammoniacal silver is reactive and should be nuetralized with table salt or a saline solution before disposal.
The protocol incubation times may vary per manufacturer guidelines. Find, read and cite all the research you need on researchgate. Reticulin and cd34 stains for the liver core biopsy of. Special stains for special circumstances some stains are ordered for special purposes when indicated by the clinical situation. The diamine silver complex is formed by a reaction between silver and. Gomori reticular fiber stain research 042016 powerpoint. Pdf a lecture in applied pathology about methods of staining and identifying connective tissues. Reticulin stains and masson trichrome stain will confirm the collagenic nature of the stroma. Before ihc, reticulin was used to differentiate sarcomas from carcinomas. Reticulin is a type of collagenous fiber that is generally argyrophilic and first recognized as a type of collagen with the advent of electron microscopy. In addition, changes in reticular pattern can be seen in some liver diseases.
The tissue is first oxidized, sensitized with iron alum, which is replaced by a silver salt. The diamine silver complex is formed by a reaction between silver andammonia. A liver biopsy stained using the reticulin demonstrating the normal hepatic plate thickness and mild steatosis. Oil red o or sudan black fat stains on fresh frozen tissue. Use only acid clean glassware and paraffin coated forceps. In humans, increased reticulin fibers are associated with many benign and malignant conditions, while. Yet, since the 1920s the definition of reticulin and studies of its distribution were based solely on silver impregnation technics. This silver staining process is known as silver impregnation. Hito reticulin optimstain kit is designed based on gordon and sweet method with nuclear counterstain, used to demonstrate reticular fibers. Learn vocabulary, terms, and more with flashcards, games, and other study tools. By highlighting these fibers, the stain helps in the assessment of the architecture of the hepatic plates, such as expansion in regenerative and neoplastic conditions, compression of plates in nodular regenerative hyperplasia, 4 and collapse of the reticulin framework in necrosis fig. Special stains, such as reticulin stain and cd34 immunostain, are very helpful in the diagnosis of well differentiated hepatocellular carcinoma hcc.
Associated with basement membranes and with fiber networks in bone marrow, lymph nodes etc. Useful in the differential diagnosis of certain type of tumors such as carcinomas, sarcomas, and lymphosarcomas. A abnormal reticulin staining pattern in one area of hcc. In the liver, such fibers are present as part of the extracellular matrix in the space of disse. Reticulin and cd34 stains for the liver core biopsy of a 71year old patient with well differentiated hepatocellular carcinoma hcc. Unusual reticulin staining pattern in welldifferentiated. Buy reticulin stain kit with gold chloride from atom scientific, the uks largest diagnostic reagent manufacture and chemical retailer for science, education and industry.
Pdf files contains the procedure plus a sample container label. Reticulin is a network of fine fibers that support tissues. Identify and visualize reticular fibers in tissue sections with the thermo scientific richardallan scientific reticulin silver stain, a gordon and sweets modification. Pdf files contains the procedure for a special stain, a procedure card, and sample container labels for the reagents. Reticular fibers, reticular fibres or reticulin is a type of fiber in connective tissue composed of type iii collagen secreted by reticular cells. Reticulin stains are commonly used in surgical pathology to assess mass lesions for the possibility of hepatocellular carcinoma. The usefulness of the reticulin stain in the differential. The loss of normal reticulin staining can help support a diagnosis of hepatocellular carcinoma, and this stain has proven to be particularly helpful on limited biopsies and fineneedle aspirates. This network acts as a supporting mesh in soft tissues such as liver, bone marrow, and the tissues and organs of the lymphatic system. The quantitative and qualitative parameters of reticulin fibers identified in 25 bm biopsies 67.
Maresch 1905 introduced bielschowskys silver impregnation technic for neurofibrils as a stain for reticulum fibers, but emphasized the nonspecifity of such procedures. On a number of occasions we have observed that reticulum fibers stain better after the ammoniacal silver has aged in the refrigerator for a few days. Problems and pitfalls in grading of bone marrow fibrosis. Gomori reticular fiber stain effect of varying developing reagents by. Intended to demonstrate reticular fibers in paraffin sections. This lack of specifity has been confirmed repeatedly. Gordon and sweets reticulin histotechnology group dst. Carcinomas have reticulin around clusters of cells. Silver impregnation methods for reticulum fibers and. In discussing bone marrow fibrosis it is important to describe the pathological tests used to define it. Demonstration of reticulin fibres in the epididymis. Giesens trichrome stain was found useful for demonstration of collagen in grade 4 fibrosis.
Reticulin fibres have little natural affinity for silver solutions so, they must be treated with potassium permanganate to. When used in a silver stain for reticulin, gold chloride functions as the. Pdf connective and mesenchymal tissues with their stains. Replace solutions after 1012 slides have been processed, one slide at a time. The reticulin stain is performed on bone biopsy sections for detecting the presence, assessing the severity and identifying specific disease. Argyrophillic cells need a reducerdeveloper to convert the invisible silver salts to a visible metallic silver.
Reticulin fibers are argyrophilic, meaning that these tissue elements will stain black with a silver solution using the aid of a chemical reducer which brings the silver into to a visible form. Argyrophillic cells can adsorb silver but cannot reduce it. Histopathologic features of hepatocellular carcinoma. We report for the first time, to our best knowledge, tacrolimus therapy associated with bone marrow fibrosis, abnormal megakaryocytosis, and dyserythopoiesis in a 17yearold male treated with tacrolimus for. Reticulin fibrosis in the bone marrow reticulin stain. These manuals are in adobe acrobat pdf format and require the acrobat reader for viewing. The silver is then reduced with a solution of formaldehyde.
Role of special stains in diagnostic liver pathology. It is worth noting that no single stain is entirely sensitive or specific, 8 and the premier stain for diagnosis remains hematoxylin and eosin. The reticulin kit serves to visualize its existence through an impregnation with a silver salt. The fibers appear black against a gray to light pink background. Accurate fibrosis grading therefore mandates standardized conditions that maintain reticulin stain quality and in particular the consistency of. Unusual reticulin staining pattern in welldifferentiated hepatocellular carcinoma by heng hong, bryan patonay and james finley topics. This kit is easy to use with simplified procedures and delivers stable and improved staining quality. Adsorb surface based process whereas absorb permeation through the tissue. Hexose sugars of reticulin demonstrated by oxidation to aldehydes.
Reticulin stain reticulin stain uses silver impregnation to detect reticulin fibers, which are made of type 3 collagen. Reticulin stains use silver and rely on the argyrophillic properties of the fibers. Reticulin stain demonstrates fibers around groups of granulosa cells, while surrounding individual theca cells. Reticular fibers crosslink to form a fine meshwork reticulin. Histopathology special stain reticulin test for surgical. These techniques rely on the impregnation of the fibres by silver ions and subsequent reduction of these silver ions to their visible metallic form. The kit is sufficient for up to 110 section slides. Identifies reticulin fibers in tissue sections liver, kidney and spleen. The reticulin nuclear fast red stain is used for the visualization of reticulin. It is used to visualize reticular fiber and used extensively in liver histo pathology.
Reticulin is a type iii collagen found in the basement membrane of many organs and provides structural integrity. To diagnose hemangiopericytoma, vascular smooth muscle, fibrosarcoma or fibrothecoma stains each cell vs. Role of special stains in diagnostic liver pathology krishna 2015. Reticulin stain highlighting the reticulin pattern in acute hepatic necrosis. Why the stain is done how the stain is interpreted pitfalls, technical aspects really reflex use of special stains special stains. Ferric ammonium sulfate acts as sensitizer, replaced by silver from dimaine silver solution. Thinwalled vascular spaces are invariably present but not in large numbers.
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